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Polymeric Nanoparticles with a Sera-Derived Coating for Efficient Cancer Cell Uptake and Killing
Daniel Nierenberg1, Orielyz Flores1, David Fox2,3
1Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, Florida 32827, United States.
ACS Omega
|March 8, 2021
Summary
Optimizing nanoparticle coatings with normal sera (NS) significantly enhances cancer drug delivery to triple-negative breast cancer cells. This novel approach improves nanoparticle uptake by cancer cells while maintaining safety and efficacy.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Cancer Therapeutics
Background:
- Nanoparticle drug delivery for cancer is inefficient.
- The protein corona on nanoparticles influences their biological interactions.
- Optimizing nanoparticle coatings can enhance cancer cell uptake.
Purpose of the Study:
- To synthesize hyperbranched polyester polymer (HBPE) nanoparticles.
- To investigate the effect of normal sera (NS) coating on nanoparticle uptake by cancer cells.
- To compare NS-coated HBPE nanoparticles with PEGylated nanoparticles for cancer drug delivery.
Main Methods:
- Synthesis of HBPE nanoparticles from diethyl malonate.
- Coating HBPE nanoparticles with normal mouse sera (NS).
- Assessing cellular uptake in MDA-MB-231 triple-negative breast cancer (TNBC) cells, endothelial, and monocytic cell lines.
Main Results:
- NS-treated HBPE nanoparticles showed higher uptake by TNBC cells compared to PEGylated nanoparticles.
- Monocyte uptake evasion was comparable between NS-treated and PEGylated HBPE nanoparticles.
- NS-treated HBPE nanoparticles demonstrated no inherent toxicity, did not promote angiogenesis, and effectively delivered paclitaxel to TNBC cells.
Conclusions:
- Normal sera coatings can enhance cancer cell-specific uptake of HBPE nanoparticles.
- NS-coated HBPE nanoparticles offer a promising platform for improved cancer drug delivery.
- This approach may lead to the discovery of new factors for enhancing nanoparticle bioavailability and tissue distribution.
