Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

14.1K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
14.1K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.3K
Atomic Force Microscopy01:08

Atomic Force Microscopy

3.8K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.8K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.4K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.4K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

11.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
11.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Three-dimensional particle localization techniques based on phase modulation and digital vortex imaging.

Optics express·2026
Same author

High-precision liquid reference Fizeau interferometry with motionless phase-shifting method and tilt-suppressed calibration method.

Optics express·2025
Same author

Generation controllable optical chain using an optical pen.

Optics letters·2024
Same author

Full-angle single-shot quantitative phase imaging based on Kramers-Kronig relations.

Optics letters·2024
Same author

Technique for enhancing the accuracy of the Rayleigh-Sommerfeld convolutional diffraction through the utilization of independent spatial sampling: publisher's note.

Optics letters·2024
Same author

Technique for enhancing the accuracy of the Rayleigh-Sommerfeld convolutional diffraction through the utilization of independent spatial sampling.

Optics letters·2024
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Nov 14, 2025

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

8.7K

Optical scanning Fourier ptychographic microscopy.

Lin Wang, Qihao Song, Hongbo Zhang

    Applied Optics
    |March 10, 2021
    PubMed
    Summary
    This summary is machine-generated.

    We developed a cost-effective active scanning optical scanning Fourier ptychographic microscopy (OSFPM) using Galvo mirrors. This method precisely reconstructs high-resolution images from multiple low-resolution scans, ideal for large samples.

    More Related Videos

    Author Spotlight: Introduction to Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays
    05:04

    Author Spotlight: Introduction to Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays

    Published on: June 13, 2023

    2.0K
    Three-dimensional Optical-resolution Photoacoustic Microscopy
    08:31

    Three-dimensional Optical-resolution Photoacoustic Microscopy

    Published on: May 3, 2011

    18.5K

    Related Experiment Videos

    Last Updated: Nov 14, 2025

    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
    12:22

    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

    Published on: August 4, 2018

    8.7K
    Author Spotlight: Introduction to Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays
    05:04

    Author Spotlight: Introduction to Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays

    Published on: June 13, 2023

    2.0K
    Three-dimensional Optical-resolution Photoacoustic Microscopy
    08:31

    Three-dimensional Optical-resolution Photoacoustic Microscopy

    Published on: May 3, 2011

    18.5K

    Area of Science:

    • Microscopy and Imaging Technologies
    • Optical Physics
    • Computational Imaging

    Background:

    • Fourier ptychographic microscopy (FPM) enables high-resolution imaging.
    • Conventional FPM systems often face limitations in cost, complexity, and scalability for large-area imaging.
    • Precise control over illumination overlap is crucial for accurate image reconstruction.

    Purpose of the Study:

    • To introduce a practical and lower-cost active scanning optical scanning Fourier ptychographic microscopy (OSFPM).
    • To demonstrate the capability of OSFPM for efficiently imaging large-sized objects.
    • To improve the control over illumination overlap in scanning FPM systems.

    Main Methods:

    • Implementation of a simple Galvo mirror-based scanning system for active beam projection.
    • Acquisition of multiple low-resolution images by projecting a laser beam in a circular pattern onto the sample.
    • Reconstruction of a high-resolution image from the captured low-resolution image dataset.

    Main Results:

    • The proposed OSFPM system achieves high-resolution image reconstruction from multiple low-resolution scans.
    • The Galvo mirror setup allows for precise control over illumination spot overlap.
    • The system demonstrates suitability for imaging larger sample areas with efficient illumination.

    Conclusions:

    • The developed active scanning OSFPM offers a practical and cost-effective solution for high-resolution microscopy.
    • This technique provides superior control over illumination overlap compared to existing scanning FPM methods.
    • OSFPM is well-suited for large-area imaging applications requiring efficient and precise illumination control.