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Simultaneous Determination of Abamectin and Closantel in Veterinary Formulation by Validated HPLC Method
Emad M Abd Elhalim1, Mohamed A Amin2, Mohamed A Ali3
1Department of Chemistry, Zagazig University, Zagazig 44519, Egypt.
Journal of Chromatographic Science
|March 12, 2021
Summary
A new high-performance liquid chromatography method accurately determines abamectin and closantel in veterinary drugs. This validated method ensures reliable quality control for these essential antiparasitic compounds.
Area of Science:
- Analytical Chemistry
- Veterinary Pharmacology
Background:
- Abamectin and closantel are crucial antiparasitic agents in veterinary medicine.
- Accurate quantification of these active ingredients in formulations is vital for efficacy and safety.
Purpose of the Study:
- To develop and validate a rapid and accurate high-performance liquid chromatography (HPLC) method.
- To enable the simultaneous determination of abamectin and closantel in veterinary formulations.
Main Methods:
- HPLC separation using an Agilent 1200 system with a UV detector and a Waters C18 column.
- Mobile phase: acetonitrile:water (80:20 v/v) adjusted to pH 3.0 with phosphoric acid.
- Flow rate: 1.5 mL/min; Injection volume: 10 μL.
Main Results:
- Linear calibration curves with high correlation coefficients (r² ≥ 0.999) for abamectin (B1a and B1b) and closantel.
- Specific retention times: Abamectin B1b (2.18 min), Abamectin B1a (3.72 min), and Closantel (5.84 min).
- Method demonstrated high accuracy and precision with relative standard deviation ≤ 2%.
Conclusions:
- The developed HPLC method is rapid, accurate, and suitable for routine quality control.
- The validated method successfully quantifies both abamectin and closantel concurrently in veterinary formulations.
