Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Blood Transfusion and Agglutination02:45

Blood Transfusion and Agglutination

12.4K
Blood transfusion is a therapeutic measure to restore the blood volume after extensive blood loss due to an accident or a medical procedure. Blood transfusion involves drawing a certain amount of blood from a suitable donor and infusing it into the recipient.
History
The history of blood transfusion dates back to the 17th century, when early attempts were made in animals. In 1818 James Blundell, a British doctor, performed the first successful human blood transfusion. Later in 1900, Karl...
12.4K
Blood Transfusion01:15

Blood Transfusion

1.3K
Blood transfusion is a critical medical procedure that saves lives and treats various medical conditions. It involves transferring blood from a donor to a recipient. This process requires a thorough understanding of the ABO blood group system and its associated antigens and antibodies.
Blood Transfusion Overview
A blood transfusion is a medical procedure used to replace blood lost due to injury, surgery, or to treat conditions such as anemia or cancer. During a transfusion, donor blood is...
1.3K
Bone Marrow Sampling and Transplants01:22

Bone Marrow Sampling and Transplants

631
Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy...
631
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

79.7K
Overview
79.7K
Tissue Transplantation01:24

Tissue Transplantation

698
Tissue transplantation is a significant medical procedure involving the transfer of cells, tissues, or organs from a donor to a recipient, with the primary aim of restoring lost functions. This procedure is crucial in treating a broad spectrum of diseases, including kidney diseases, liver failure, heart disease, and certain types of cancers.
The Biology of Tissue Transplantation
The biology of tissue transplantation hinges on the Major Histocompatibility Complex (MHC) molecules. These molecules...
698
Formation of the Platelet Plug01:22

Formation of the Platelet Plug

8.0K
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
8.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Defining intensive care status in metastatic lung cancer patients: A French survey.

Respiratory medicine and research·2026
Same author

Anti-CD19 CAR T-cell therapy in relapsed/refractory T-cell/histiocyte-rich large B-cell lymphoma: insights from the French DESCAR-T registry, a LYSA study.

Haematologica·2026
Same author

Characterisation of the Novel HLA-C*05:315 Allele by Sequencing-Based Typing.

HLA·2026
Same author

Characterisation of the Novel HLA-B*35:08:18 Allele by Sequencing-Based Typing.

HLA·2026
Same author

Characterisation of the Novel HLA-B*27:301 Allele by Sequencing-Based Typing.

HLA·2026
Same author

Outcome of patients with mantle cell lymphoma after failure of anti-CD19 CAR T-cell therapy: a DESCAR-T study by LYSA Group.

Blood advances·2025

Related Experiment Video

Updated: Nov 13, 2025

Routine Screening Method for Microparticles in Platelet Transfusions
09:49

Routine Screening Method for Microparticles in Platelet Transfusions

Published on: January 31, 2018

16.2K

Platelet transfusion refractoriness and anti-HLA immunization.

Lucie Blandin1, Aurore Dougé1,2, Amandine Fayard3

  • 1Histocompatibility and Immunogenetics Laboratory, Clermont-Ferrand University Hospital, Clermont-Ferrand, France.

Transfusion
|March 12, 2021
PubMed
Summary

Platelet transfusion refractoriness (PTR) management can be improved by identifying anti-human leukocyte antigen (HLA) antibodies in patients. Selecting platelet donors negative for specific HLA antigens effectively addresses PTR in immunized patients.

Keywords:
MFI thresholdanti-HLA immunizationplatelet transfusion refractoriness

More Related Videos

Treatment of Platelet Products with Riboflavin and UV Light: Effectiveness Against High Titer Bacterial Contamination
10:32

Treatment of Platelet Products with Riboflavin and UV Light: Effectiveness Against High Titer Bacterial Contamination

Published on: August 24, 2015

13.9K
Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System
12:40

Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

Published on: December 7, 2012

29.2K

Related Experiment Videos

Last Updated: Nov 13, 2025

Routine Screening Method for Microparticles in Platelet Transfusions
09:49

Routine Screening Method for Microparticles in Platelet Transfusions

Published on: January 31, 2018

16.2K
Treatment of Platelet Products with Riboflavin and UV Light: Effectiveness Against High Titer Bacterial Contamination
10:32

Treatment of Platelet Products with Riboflavin and UV Light: Effectiveness Against High Titer Bacterial Contamination

Published on: August 24, 2015

13.9K
Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System
12:40

Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

Published on: December 7, 2012

29.2K

Area of Science:

  • Immunology
  • Transfusion Medicine
  • Hematology

Background:

  • Platelet transfusion refractoriness (PTR) is a frequent complication in patients requiring multiple transfusions.
  • An unsatisfactory post-transfusion platelet count increment defines PTR.
  • Current management strategies for PTR are diverse.

Purpose of the Study:

  • To demonstrate the efficacy of detecting and identifying anti-human leukocyte antigen (HLA) antibodies in recipients for managing PTR.
  • To establish a threshold for antibody detection (3000 mean fluorescence intensity - MFI).
  • To guide the selection of compatible platelet donors based on patient immunization.

Main Methods:

  • Detection and identification of anti-HLA antibodies in recipients.
  • Utilizing a mean fluorescence intensity (MFI) threshold of 3000 for antibody identification.
  • Screening potential platelet donors for the absence of immunizing HLA antigens.

Main Results:

  • Successful identification of anti-HLA antibodies in PTR patients using the defined MFI threshold.
  • Demonstrated efficacy in selecting HLA-compatible donors.
  • Improved outcomes in patients receiving transfusions from specifically selected donors.

Conclusions:

  • Detection of anti-HLA antibodies with an MFI threshold of 3000 is an effective strategy for managing PTR.
  • Identifying and avoiding donor antigens against which the patient is immunized is crucial.
  • This approach enhances transfusion efficacy in patients with PTR.