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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
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EasyMAP: A user-friendly online platform for analyzing 16S ribosomal DNA sequencing data.
Yuan-Mao Hung1, Tzu-Pin Lu2, Mong-Hsun Tsai3
1Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei, Taiwan.
New Biotechnology
|March 12, 2021
Summary
EasyMAP is a new online platform for analyzing 16S ribosomal DNA sequencing data. It provides a user-friendly interface for microbial community analysis and gene function prediction.
Area of Science:
- Microbiology
- Bioinformatics
- Computational Biology
Background:
- Next-generation sequencing has advanced microbial 16S ribosomal DNA (rDNA) research.
- Analyzing 16S rDNA sequences reveals bacterial and archaeal composition.
- Existing analytical tools often lack user-friendly graphical interfaces.
Purpose of the Study:
- To develop a comprehensive and easy-to-use online platform for 16S rDNA sequencing data analysis.
- To integrate popular bioinformatics pipelines into a single, accessible tool.
- To provide step-by-step guidance for various analytical tasks.
Main Methods:
- Development of the Easy Microbiome Analysis Platform (EasyMAP).
- Integration of QIIME2, LefSe, and PICRUSt pipelines.
- Inclusion of temporal profiling and longitudinal analysis capabilities.
Main Results:
- EasyMAP offers a user-friendly graphical interface for 16S rDNA data analysis.
- The platform enables quality checks, differential abundance analysis, and microbial gene function prediction.
- Temporal and longitudinal analyses are supported.
Conclusions:
- EasyMAP is a valuable, user-friendly tool for comprehensive 16S rDNA sequencing data analysis.
- The platform democratizes advanced microbiome analysis.
- It is freely available online with documentation.
