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Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
Published on: September 25, 2018
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Multiplexed digital polymerase chain reaction as a powerful diagnostic tool.
Martina Gaňová1, Haoqing Zhang2, Hanliang Zhu2
1Central European Institute of Technology, Brno University of Technology, 612 00, Brno, Czech Republic.
Biosensors & Bioelectronics
|March 19, 2021
Summary
Digital polymerase chain reaction (dPCR) multiplexing enables simultaneous detection of related DNA sequences. This review covers dPCR techniques, applications, and future potential in research and clinical settings.
Area of Science:
- Biotechnology
- Molecular Biology
- Analytical Chemistry
Background:
- Digital polymerase chain reaction (dPCR) offers advanced capabilities for analyzing complex DNA mixtures.
- Continuous innovation in microfluidics and fabrication drives dPCR improvements.
Purpose of the Study:
- To review current dPCR multiplexing techniques.
- To discuss their applications in research and clinical settings.
- To evaluate their strengths, weaknesses, and future prospects.
Main Methods:
- Review of dPCR techniques including sample compartmentalization, droplet-based, and chip-based systems.
- Analysis of multiplexing strategies in laboratory and clinical contexts.
- Evaluation of method performance based on sample type and required precision.
Main Results:
- dPCR multiplexing effectively detects and quantifies closely related DNA sequences.
- Various dPCR platforms show distinct advantages and limitations for different applications.
- Recent advancements highlight the growing utility of dPCR in diverse biological analyses.
Conclusions:
- dPCR multiplexing is a powerful tool for complex genetic analysis.
- Ongoing technological advancements promise expanded applications in diagnostics and research.
- Further development is expected to enhance precision and broaden the scope of dPCR capabilities.
