Production of monoclonal antibodies to Sp2/O-Ag 14 myeloma cells

The hybridoma technology developed by Kohler and Milstein allows the obtention of antibodies of a single specificity (i.e. monoclonal antibodies). This methodology was first introduced in the Immunology Laboratory of the Department of Microbiology at the Medical Sciences Campus (MSC), University of Puerto Rico (UPR) in 1986. The production of monoclonal antibodies to Sp2/O-Ag 14 reported herein describes the procedure used and is of importance because it constitutes the first work on monoclonal antibodies performed at our institution and in Puerto Rico.