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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
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Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
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Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
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Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
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Single virus inductively coupled plasma mass spectroscopy analysis: A comprehensive study.

Claude Degueldre1

  • 1Engineering Department, Lancaster University, Lancaster, LA1 4YW, UK.

Talanta
|March 28, 2021
PubMed
Summary

This study adapts single particle ICP-MS (SP-ICP-MS) for identifying and counting single viruses (SV). The method uses elemental ion detection for rapid viral quantification and characterization.

Keywords:
Individual virus analysisSP ICP-MSVirus countingVirus identificationsingle virus

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Area of Science:

  • Analytical Chemistry
  • Biophysics
  • Nanotechnology

Background:

  • Single particle inductively coupled plasma mass spectrometry (SP-ICP-MS) enables nanoparticle characterization.
  • Analysis of biological entities at the single-unit level is a growing field.

Purpose of the Study:

  • To adapt SP-ICP-MS for the identification and counting of single viruses (SV).
  • To establish elemental ratios for virus characterization and quantification.

Main Methods:

  • Utilizing high-resolution multi-channel sector field (MC SF) ICP-MS in SV detection mode.
  • Counting master ions (C, N) and key ions (P, S) for viral analysis.
  • Investigating time scan and fixed mass analysis for SARS-CoV-2 and bacteriophage T5.

Main Results:

  • Demonstrated counting of 2-500 viral units within 20 seconds.
  • Established N/C, P/C, and S/C molar ratios for characterizing viral species.
  • Quantified the number concentration of single viruses.

Conclusions:

  • SP-ICP-MS is a viable method for single virus analysis.
  • High-resolution SF ICP-MS is recommended for optimal performance.
  • Further optimization with anaerobic/aerobic atmospheres can enhance quadrupole ICP-MS analysis.