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2D titanium carbide nanosheets based fluorescent aptasensor for sensitive detection of thrombin
Hongyuan Cui1, Xueqi Fu2, Lin Yang3
1School of Life Sciences, Jilin University, Changchun, 130012, PR China; Key Laboratory of Physics and Technology for Advanced Batteries (Ministry of Education), College of Physics, Jilin University, Changchun, 130012, PR China.
Talanta
|March 28, 2021
Summary
A new aptasensor using titanium carbide (Ti3C2) MXene detects thrombin with high sensitivity. This fluorescence resonance energy transfer (FRET) biosensor shows promise for accurate clinical diagnosis.
Area of Science:
- Materials Science
- Nanotechnology
- Biomedical Engineering
Background:
- Two-dimensional titanium carbides (MXenes) offer unique electrical and optical properties for biosensing.
- Developing sensitive and specific biosensors is crucial for early disease detection and monitoring.
Purpose of the Study:
- To construct a highly sensitive and specific fluorescence resonance energy transfer (FRET) aptasensor for quantitative thrombin detection.
- To utilize single-layer Ti3C2 MXene as a platform for enhanced aptasensor performance.
Main Methods:
- Fabrication of a FRET aptasensor using Ti3C2 MXene and a dye-labeled thrombin-binding aptamer (TBA).
- Exploitation of FRET between the dye and Ti3C2 for fluorescence quenching.
- Induction of fluorescence recovery upon thrombin binding to TBA and subsequent detachment from Ti3C2.
Main Results:
- The Ti3C2 MXene-based FRET aptasensor achieved a low detection limit of 5.27 pM for thrombin.
- The aptasensor demonstrated high sensitivity and specificity, with aptamer HD22 showing optimal performance.
- Accurate detection of thrombin in human serum was achieved, validating real-world applicability.
Conclusions:
- Ti3C2 MXene is a promising material for developing advanced FRET aptasensors.
- The developed aptasensor offers a sensitive and specific method for thrombin quantification.
- This technology holds significant potential for clinical diagnosis and real-world biomedical applications.
