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Amino acid sequence of porcine heart fumarase
J C Sacchettini1, M W Frazier, D C Chiara
1Department of Biological Chemistry, Washington University School of Medicine, St. Louis, Missouri 63110.
Biochemical and Biophysical Research Communications
|May 31, 1988
Abstract:
The complete amino acid sequence of porcine heart fumarase (EC 4.2.1.2) has been determined from peptides produced by cyanogen bromide, endoproteinase Arg-C, S. aureus V8 protease, and trypsin. The enzyme is a tetramer of identical subunits with Mr = 50,015 and composed of 466 amino acid residues. Porcine heart fumarase displays 96% identity to human liver fumarase. Prediction of the secondary structural elements of porcine fumarase indicate that the enzyme contains a large amount of alpha helix with very little beta structure.