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Transcriptome-wide quantification of double-stranded RNAs in live mouse tissues by dsRIP-Seq

Yimeng Gao1,2, Shirui Chen3, Stephanie Halene1,2

  • 1Section of Hematology, Yale Cancer Center and Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520, USA.

STAR Protocols
|March 29, 2021
PubMed
Summary

This study introduces a dsRNA immunoprecipitation and sequencing (dsRIP-Seq) protocol to measure double-stranded RNAs (dsRNAs) in mouse tissues. This method helps understand aberrant dsRNAs that trigger harmful innate immune responses.

Keywords:
Cell BiologyImmunologyModel OrganismsRNA-seqSequencing

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