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BBN-driven urinary bladder cancer mouse model.

Jelena Korac-Prlic1, Marina Degoricija1, Katarina Vilović2

  • 1Laboratory for Cancer Research, University of Split School of Medicine, Split, Croatia.

Methods in Cell Biology
|March 31, 2021
PubMed
Summary

This study details a protocol for inducing bladder cancer (BC) in mice using N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN). This reliable mouse model aids in understanding BC biology and developing new treatments.

Keywords:
BBNBladder cancerChemical carcinogenesisMIBCMouse modelSmoking

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Area of Science:

  • Oncology
  • Animal Models
  • Carcinogenesis

Background:

  • Urinary bladder cancer (BC) accounts for 3% of new cancer diagnoses and 2% of cancer deaths annually.
  • Effective preclinical models are crucial for advancing BC research.
  • The N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN) model closely mimics human muscle-invasive bladder cancer.

Purpose of the Study:

  • To present a detailed protocol for inducing bladder cancer in mice using BBN.
  • To establish a reproducible model for studying BC pathogenesis.
  • To facilitate the discovery of novel diagnostic and therapeutic strategies for bladder cancer.

Main Methods:

  • Administration of 0.05%-0.1% BBN in drinking water to 6-8 week old mice for 12 weeks.
  • Tumor development monitored 8 weeks post-BBN treatment.
  • Histopathological assessment of lesions via hematoxylin and eosin staining.
  • Analysis of RNA, DNA, and proteins from urinary bladder tissue.

Main Results:

  • BBN administration reliably induces high-grade, invasive bladder tumors in mice.
  • Tumor histology and genetic alterations resemble human muscle-invasive bladder cancer.
  • Tumor incidence and differentiation are influenced by mouse strain, gender, BBN concentration, and protocol timeline.

Conclusions:

  • The described BBN-induced mouse model is a valuable tool for bladder cancer research.
  • This model enables comprehensive molecular analysis of BC.
  • Utilizing this model can accelerate biomedical discoveries and clinical advancements in bladder cancer treatment.