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Diffusion-Convection Hybrid Microfluidic Platform for Rapid Antibiotic Susceptibility Testing.

Ann V Nguyen1, Morteza Azizi1, Mohammad Yaghoobi1

  • 1Department of Food Science, College of Agricultural and Life Sciences, Cornell University, Stocking Hall, Ithaca, New York 14853, United States.

Analytical Chemistry
|March 31, 2021
PubMed
Summary

This study introduces a rapid microfluidic platform for antibiotic susceptibility testing (AST). The new method determines minimum inhibitory concentrations (MICs) in 4-5 hours, significantly faster than traditional assays.

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Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Clinical Microbiology

Background:

  • Conventional antibiotic susceptibility testing (AST) methods are time-consuming (24-72 hours) and labor-intensive.
  • There is a need for faster and more efficient AST methods to guide clinical treatment decisions.
  • Microfluidic platforms offer potential for miniaturization and rapid assay development.

Purpose of the Study:

  • To develop and validate a novel microfluidic platform for rapid antibiotic susceptibility testing.
  • To generate a precise antibiotic concentration gradient within the microfluidic device.
  • To accurately determine minimum inhibitory concentrations (MICs) using bacterial metabolic activity.

Main Methods:

  • A microfluidic chip was designed to create a serial antibiotic concentration gradient using diffusion and mass convection.
  • Bacterial metabolism was monitored via resazurin fluorescence to assess antibiotic effects.
  • The platform was tested using human isolates and clinically relevant antibiotics, comparing results to broth microdilution.

Main Results:

  • The microfluidic platform successfully generated stable antibiotic concentration gradients, validated by simulation and experiment.
  • Minimum inhibitory concentrations (MICs) were determined within 4-5 hours.
  • A high agreement rate (94%) was observed between on-chip MICs and the reference broth microdilution method.

Conclusions:

  • The developed microfluidic platform provides a rapid and reliable method for antibiotic susceptibility testing.
  • This technology has the potential to significantly reduce turnaround time for AST results in clinical settings.
  • The platform's design is adaptable for various molecules and assay conditions.