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Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
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AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
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The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
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Laser-Induced Fluorescence Emission L.I.F.E. as Novel Non-Invasive Tool for In-Situ Measurements of Biomarkers in Cryospheric Habitats
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A Compact LIF Spectrometer for in-Field Operation in Polar Environments.

Lorenzo Palombi1, Valentina Raimondi1

  • 1"Nello Carrara" Institute of Applied Physics-National Research Council, I-50019 Sesto Fiorentino, FI, Italy.

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|April 3, 2021
PubMed
Summary
This summary is machine-generated.

A new compact laser-induced fluorescence (LIF) spectrometer, SFIDA-405, was developed for polar field studies. This portable instrument successfully analyzed biological soil crusts in the Arctic and Antarctic.

Keywords:
biological soil crustslaser-induced fluorescencemicroorganismspolar environmentspectrometer

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Area of Science:

  • Environmental science
  • Spectroscopy
  • Polar research

Background:

  • In-situ analysis of biological soil crusts in polar regions is challenging.
  • Existing spectroscopic instruments may not be suitable for harsh, remote environments.

Purpose of the Study:

  • To develop and test a compact, portable laser-induced fluorescence (LIF) spectrometer for in-field operation in polar environments.
  • To assess the instrument's capability for studying biological soil crusts.

Main Methods:

  • A novel compact LIF spectrometer (SFIDA-405) utilizing 405 nm excitation was designed.
  • The instrument features an optical-fiber coupled measurement head and operates on battery power (<1.6 kg).
  • Field campaigns in the Arctic and Antarctic were conducted for instrument validation.

Main Results:

  • The SFIDA-405 prototype demonstrated successful in-field operation in polar conditions.
  • It achieved a limit of detection for chlorophyll better than 5 ng/cm2.
  • The first in-situ LIF spectra of Antarctic biological soil crusts were acquired.

Conclusions:

  • The SFIDA-405 is a viable, portable tool for in-situ spectroscopic analysis in extreme polar environments.
  • The instrument shows potential for diverse applications beyond biological soil crust studies.
  • This work represents a significant advancement in polar field spectroscopy.