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Updated: Nov 9, 2025
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Reconstruction of 3-Dimensional Histology Volume and its Application to Study Mouse Mammary Glands
Published on: July 26, 2014
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3D reconstruction and histopathological analyses on murine corporal body
Daiki Hashimoto1, Mizuki Kajimoto1, Yuko Ueda2
1Department of Developmental Genetics Institute of Advanced Medicine Wakayama Medical University Wakayama Japan.
Reproductive Medicine and Biology
|April 14, 2021
Summary
Aging causes changes in penile structure, leading to erectile dysfunction (ED). Aged corpus cavernosum shows ectopic chondrocytes and altered SOX9/Notch signaling, potentially contributing to ED.
Area of Science:
- Urology
- Histology
- Regenerative Medicine
Background:
- Erectile dysfunction (ED) prevalence increases with age.
- The complex 3D structure of penile sinusoids in the corpus cavernosum (CC) is not fully understood.
- Penile abnormalities contribute to ED, necessitating detailed structural analysis.
Purpose of the Study:
- To analyze the 3D histological structure of murine corpus cavernosum (CC) sinusoids.
- To compare CC structure and chondrogenic signaling between young and aged mice.
- To investigate the role of SOX9 and RBPJK in age-related penile changes.
Main Methods:
- 3D reconstruction of serial sections from murine CC.
- Histological analysis comparing 2-month-old (young) and 14-month-old (aged) CC.
- Immunohistochemical staining for SOX9 and RBPJK in aged CC.
Main Results:
- Sinusoids are prominent in the outer CC regions near the tunica albuginea.
- Aged CC samples exhibit ectopic chondrocytes in these outer regions.
- SOX9 expression is upregulated, while RBPJK expression is downregulated in aged CC.
Conclusions:
- Prominent sinusoids in the outer CC may play a crucial role in erection.
- Aging appears to induce ectopic chondrogenesis in the CC.
- Altered SOX9 and Notch signaling (RBPJK) may contribute to age-related penile dysfunction.
