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High-resolution anion exchange chromatography of the glucocorticoid receptor from WEHI-7 cells
D J Gruol1, N F Campbell, S Bourgeois
1Salk Institute for Biological Studies, San Diego, CA 92138.
Journal of Steroid Biochemistry
|January 1, 1988
Abstract:
A simple refinement of the current methods of DEAE chromatography of steroid receptors has been developed which takes advantage of the characteristics of Fast Flow DEAE Sepharose (Pharmacia). The approach provides a convenient and inexpensive means to carry out high-resolution chromatography of the glucocorticoid receptor. Using this method, at least five separate species of receptor have been detected within the single so-called "low-salt" peak normally seen using the current methods of receptor anion exchange chromatography.