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Heterochromatic Nonlinear Optical Responses in Upconversion Nanoparticles for Super-Resolution Nanoscopy
Chaohao Chen1, Baolei Liu1, Yongtao Liu1
1Institute for Biomedical Materials & Devices (IBMD), Faculty of Science, University of Technology Sydney, Sydney, NSW, 2007, Australia.
Advanced Materials (Deerfield Beach, Fla.)
|April 17, 2021
Summary
Upconversion nanoparticles (UCNPs) enable faster, more stable super-resolution nanoscopy by engineering their light emission. This method achieves 40 nm resolution, overcoming limitations of current laser scanning microscopy techniques.
Area of Science:
- Nanotechnology
- Microscopy
- Optical Engineering
Background:
- Super-resolution microscopy requires advanced point spread function (PSF) engineering.
- Current laser scanning microscopy methods face challenges like low speed, poor stability, and operational complexity.
Purpose of the Study:
- To develop a faster and more stable super-resolution nanoscopy technique using upconversion nanoparticles (UCNPs).
- To improve imaging quality and overcome limitations of existing super-resolution methods.
Main Methods:
- Utilized diverse emission responses of UCNPs with a doughnut-shaped scanning excitation beam.
- Collected four-photon emission for high-frequency information and two-photon emission for low-frequency information.
- Employed Fourier-domain heterochromatic fusion for optimized image quality.
Main Results:
- Achieved a spatial resolution of 40 nm (1/24th of the excitation wavelength).
- Demonstrated simultaneous collection of low- and high-frequency image information.
- Enabled super-resolution imaging with improved speed and stability.
Conclusions:
- Upconversion nanoparticles offer a novel approach for super-resolution nanoscopy.
- The developed method provides a new scope for nonlinear multi-color emitting probes in advanced imaging.
