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Updated: Nov 8, 2025

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
Published on: December 29, 2021
Intracellular enzyme-powered DNA circuit with a tunable amplifier for miRNA imaging
Yingjie Yu1, Lidan Li, Guobao Li
1Institute of Translational Medicine, Department of Otolaryngology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Health Science Center, Shenzhen 518035, China. nieguohui@email.szu.edu.cn.
Abstract:
We describe an intracellular enzyme-powered DNA circuit probe with a tunable amplifier for sensitive and selective detection of miRNA. This approach has been successfully applied for in situ miRNA-21 fluorescence imaging in live cells. Also, we used chemicals to elevate the APE1 expression level rendering a tunable amplification strength for more flexible imaging applications.
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