A Selective Culture Medium for Screening Carbapenem Resistance in Pseudomonas spp
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Updated: Nov 8, 2025
Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
Published on: June 30, 2016
Patrice Nordmann1,2,3,4, Claudine Fournier1, Laurent Poirel1,2,3
1Medical and Molecular Microbiology, Department of Medicine, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland.
Abstract:
The SuperCP medium containing meropenem (2 mg/L) was evaluated for screening of carbapenem-resistant Pseudomonas species. It was evaluated using 29 meropenem-susceptible and 56 meropenem-nonsusceptible Pseudomonas-like clinical isolates, the latter exhibiting a variety of carbapenem-resistance mechanisms. Its sensitivity and specificity of detection were found to be 91% and 100%, respectively. By testing spiked stools, an excellent performance of the medium was also observed for detection of carbapenem-resistant Pseudomonas aeruginosa, with a lowest detection limit ranging from 100 to 102 CFU/mL. This screening medium provides the opportunity to select carbapenem-resistant Pseudomonas and Pseudomonas-related isolates regardless of their resistance mechanism.
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