A Polymer-Based Matrix for Effective SALDI Analysis of Lipids

Surface-assisted laser desorption/ionization (SALDI) has become an attractive branch of matrix-assisted laser desorption/ionization (MALDI) and has been successfully applied for the detection of small molecules due to the lack of the interference of matrix. Herein, the low-cost and highly accessible polyvinylidene fluoride (PVDF) was modified using a facile alkali treatment and investigated as a SALDI matrix. The modified PVDF has a strong optical absorption and can be applied as a dual-mode substrate for both SALDI MS and SALDI imaging analysis. Modified PVDF powder showed superior performance in SALDI MS analysis of lipids, with good reproducibility, high sensitivity, and low background interference, especially for triacylglycerols (TAGs) and fatty acids. Additionally, the lipids in raw and extracted serum were both successfully determined with modified PVDF powder. A modified PVDF membrane (m-PVDF-m) showed excellent ability in lipids imaging in tissues due to its flat surface, mass signal enhancement, and elimination of matrix coating. The distribution of several TAGs and cholesteryl esters on mouse kidney section was presented by SALDI imaging directly on m-PVDF-m. These results demonstrated that modified PVDF materials presented exciting opportunities as matrices for the first time in SALDI MS acquisition and SALDI imaging.