Stimulus-coupled release of amino acids from cerebellar granule cells in culture

Cerebellar cultures greatly enriched in excitatory granule neurons were depolarized by exposure to either elevated K+ or veratrine. Stimulation of the release of not only Glu, but also of certain amino acids, including Gly, Ala and Ser, was observed. The effect was specific, as depolarization did not induce the release of all the estimated amino acids or of lactate dehydrogenase. In comparison with the characteristics of the evoked release of Glu, those of the responsive neutral amino acids were similar in terms of Ca2+-dependence, but differences were also noted. Thus, upon stimulation, the relative rise was smaller than for Glu and the degree of depolarization causing maximal release was lower. The questions of whether stimulus-coupled release of the non-transmitter amino acids from granule cells may play a neuromodulatory role in the cerebellum is discussed.