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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

627
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
627
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

889
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
889
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

449
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
449
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

1.2K
A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
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Related Experiment Video

Updated: Nov 7, 2025

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
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Fast Gating for Raman Spectroscopy.

Andrea Chiuri1, Federico Angelini1

  • 1Diagnostic and Metrology Laboratory, FSN-TECFIS-DIM Nuclear Fusion and Safety Technologies Department, ENEA Via Enrico Fermi 45, 00044 Frascati, Italy.

Sensors (Basel, Switzerland)
|April 30, 2021
PubMed
Summary

Fast gating in Raman spectroscopy effectively suppresses unwanted fluorescence by utilizing rapid timing. This technique helps improve signal quality by distinguishing between fluorescence and Raman signals based on their lifetimes.

Keywords:
ICCDKerrRamanSPADtime-gating

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Area of Science:

  • Spectroscopy
  • Analytical Chemistry
  • Physical Chemistry

Background:

  • Fluorescence often interferes with Raman spectroscopy, obscuring valuable sample information.
  • Existing techniques like CARS, SERS, and SERDS aim to enhance Raman signals or cancel fluorescence.
  • Rejecting fluorescence in traditional Raman spectroscopy remains a challenge due to picosecond timescales.

Purpose of the Study:

  • To discuss the utility of time-domain fast gating for fluorescence rejection in Raman spectroscopy.
  • To evaluate the performance of different fast gating techniques reported in the literature.

Main Methods:

  • Fast gating (<1 ns) in the time domain to discriminate fluorescence from Raman signals.
  • Analysis of techniques including optical Kerr cells, intensified Charge-Coupling Devices (ICCDs), and Single Photon Avalanche Photodiode (SPAD) systems.
  • Review of literature data on the performance of these fast gating methods.

Main Results:

  • Fast gating allows for the selective cutoff of fluorescence based on its lifetime relative to the Raman signal.
  • The effectiveness of gating depends on fluorescence lifetime and laser pulse duration.
  • Performance data for optical Kerr cells, ICCDs, and SPAD systems are discussed.

Conclusions:

  • Time-domain fast gating is a valuable approach for improving Raman spectroscopy by minimizing fluorescence interference.
  • The choice of technique (optical Kerr cells, ICCDs, SPADs) depends on specific experimental requirements and performance needs.
  • Further research and application of these methods can enhance the reliability of Raman spectroscopic analysis.