Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Nov 6, 2025

Fixed Target Serial Data Collection at Diamond Light Source
06:19

Fixed Target Serial Data Collection at Diamond Light Source

Published on: February 26, 2021

3.5K

Integrated sample-handling and mounting system for fixed-target serial synchrotron crystallography.

Gabrielle Illava1, Richard Jayne2, Aaron D Finke3

  • 1Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA.

Acta Crystallographica. Section D, Structural Biology
|May 5, 2021
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Using a Syringe Service Program-Based Community Advisory Board to Expand HIV Testing, Overdose Prevention, and PrEP Linkage in a Southern Black Neighborhood.

Journal of racial and ethnic health disparities·2026
Same author

Customizing Ionic Micelles by Dynamic Coassembly of Sequence-Defined Peptoid Block Copolymers.

Macromolecules·2026
Same author

Simulating neutron protein crystallography experiments: applications to the development of the NMX instrument at ESS.

Acta crystallographica. Section D, Structural biology·2026
Same author

TRIM24 in Human Cancers: A Dual-Function Oncoprotein, Regulatory Mechanisms, and Emerging Therapeutic Strategies.

International journal of cancer·2026
Same author

Polarization increases nuclear stiffness in macrophages despite reduction in lamin A/C levels.

npj biological physics and mechanics·2026
Same author

Ice-free vitrification of embryos and its impact on post-warming outcomes.

Scientific reports·2026

A new integrated system enhances serial synchrotron crystallography (SSX) by improving microcrystal handling and data collection. This technology supports structure-function studies and drug discovery with ultralow background scatter and optimized sample preparation.

Area of Science:

  • Structural Biology
  • Biophysics
  • Drug Discovery

Background:

  • Serial synchrotron crystallography (SSX) is crucial for biomolecular structure-function studies and drug discovery, but requires efficient handling of microcrystals.
  • Existing methods face challenges with crystal dispersion, hydration, and data collection, limiting throughput and data quality.

Purpose of the Study:

  • To develop and present an integrated system that overcomes common limitations in microcrystal preparation and data collection for SSX.
  • To enhance the efficiency and reliability of structure-function studies and drug discovery using small crystals.

Main Methods:

  • Development of an integrated system including ultralow background-scatter sample holders, a sample-loading station, and a humid glovebox.
  • Sample holders feature thin-film supports optimized for crystal dispersion, liquid removal, rapid cooling, and minimal background scatter.
Keywords:
high throughputprotein crystallographysample supportsample-delivery systemserial crystallographyserial synchrotron crystallography

More Related Videos

The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble
06:50

The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble

Published on: June 5, 2021

3.8K
An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
07:55

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

Published on: July 6, 2019

13.5K

Related Experiment Videos

Last Updated: Nov 6, 2025

Fixed Target Serial Data Collection at Diamond Light Source
06:19

Fixed Target Serial Data Collection at Diamond Light Source

Published on: February 26, 2021

3.5K
The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble
06:50

The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble

Published on: June 5, 2021

3.8K
An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
07:55

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

Published on: July 6, 2019

13.5K
  • Sample-loading station enables controlled humidity, suction-based liquid removal, cryoprotection, and sealing for room-temperature data collection.

    • Main Results:

    • The integrated system facilitates the preparation of well-dispersed, hydrated, and isomorphous microcrystals.
    • Ultralow background-scatter sample holders allow extensive oscillation data collection without obstruction or saturating Bragg peaks.
    • The system supports both cryogenic and room-temperature data collection, enhancing flexibility and compatibility with existing infrastructure.

    Conclusions:

    • The developed integrated SSX system significantly improves microcrystal handling, preparation, and data collection efficiency.
    • This technology is valuable for structure-function studies, drug discovery, and various single-crystal and few-crystal diffraction experiments.
    • The system's ease of use, flexibility, and optimized performance make it a powerful tool for crystallographic research.