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Cell Co-culture Patterning Using Aqueous Two-phase Systems
Published on: March 26, 2013
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Aqueous Two-Phase Systems and Microfluidics for Microscale Assays and Analytical Measurements
Tasdiq Ahmed1, Cameron Yamanishi1, Taisuke Kojima1
1Walter H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory School of Medicine, Atlanta, Georgia 30332, USA;
Summary
Aqueous two-phase systems (ATPS) are formed by phase separation in water, enabling purification and analysis of biomolecules. These biocompatible systems are advancing microscale applications and offering insights into cellular processes.
Area of Science:
- Biochemistry
- Materials Science
- Chemical Engineering
Background:
- Phase separation is a natural phenomenon observed across various substances, including polymers, salts, and biomacromolecules.
- Aqueous two-phase systems (ATPS) are formed when water-soluble polymers or salts induce separation into distinct aqueous phases.
Purpose of the Study:
- To review the properties, handling, and diverse applications of aqueous two-phase systems (ATPS).
- To highlight recent advancements in the fundamental understanding and commercialization of ATPS.
Main Methods:
- Review of existing literature on ATPS properties, applications, and advancements.
- Discussion of ATPS utility in purification, analytical biochemistry, and microscale technologies.
Main Results:
- ATPS are effective for purifying proteins, nucleic acids, viruses, cells, and emerging biomolecules like extracellular vesicles and biopharmaceuticals.
- Applications extend to analytical biochemistry for quantifying molecular interactions and monitoring biological activity.
- ATPS enable miniaturized applications including droplet microfluidics, immunoassays, and cell patterning due to their microscale properties and biocompatibility.
Conclusions:
- Aqueous two-phase systems (ATPS) offer versatile solutions for separation, purification, and analysis in biological and chemical contexts.
- The biocompatibility and microscale adaptability of ATPS position them for innovative applications in diagnostics and fundamental research, including intracellular phase separation and origin-of-life studies.
