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Updated: Nov 6, 2025
Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
Published on: August 5, 2011
Dimethylacetamide in Rooster Semen Cryopreservation
N L S Gatti1, C D Corcini2, J S Filho3
1Pos-Graduacao em Veterinaria, Faculdade de Veterinaria, Universidade Federal de Rio Grande, Rio Grande, Brasil.
Background:
Sperm cryopreservation of cockerels is a major challenge, and so far there is no adequate information to enable commercial use of frozen semen.
Objective:
To test the toxicity of dimethylacetamide (DMA).
Materials And Methods:
DMA was added at 3%, 6%, 9% and 12% to the freezing diluent, and maintained for equilibration with the semen sample for 1 min, 3 min, 5 min, 7 min and 9 min prior to freezing. Thawed semen was evaluated for kinetic characteristics by computer-assisted semen analysis (CASA) and for structural and functional properties by flow cytometry (plasma membrane rupture, mitochondrial functionality and plasma membrane functionality).
Results And Conclusion:
The addition of 6% DMA for 3-min equilibration resulted in the highest total and progressive motility, 42.0% and 36.9%, respectively. The point of intersection between a good protection and low plasma membrane rupture was obtained with the addition of 6% of DMA for 3-min equilibration with the rooster semen.
