Towards highly specific aptamer-affinity monolithic column by efficient UV light-initiated polymerization in...

Area of Science:

• Analytical Chemistry
• Materials Science
• Biotechnology

Background:

• Traditional methods for preparing aptamer-affinity monolithic columns are time-consuming and complex.
• Efficient preparation of these affinity columns is crucial for various analytical applications.

Purpose of the Study:

• To develop a rapid, facile, and efficient strategy for preparing aptamer-based hybrid affinity monolithic columns.
• To optimize a one-pot, room-temperature method utilizing simultaneous UV-initiated polymerization and "thiol-ene" click chemistry.

Main Methods:

• Simultaneous UV light-initiated free-radical polymerization and "thiol-ene" click reaction in a one-pot system.
• Optimization of photo-initiated polymerization recipe for ochratoxin A (OTA) detection.
• Characterization using SEM, FTIR, EDS, and pore size distribution analysis.
• Evaluation of specific recognition performance and nonspecific adsorption.

Main Results:

• Polymerization reaction completed in just 7 minutes, a 100-fold reduction compared to thermal polymerization.
• Achieved higher aptamer reaction efficiency (>88.2%) and superior selectivity for OTA.
• Demonstrated significantly lower nonspecific adsorption (1/25th of traditional methods).
• Successfully applied to beer and red wine samples with high recovery yields (99.7%–101.2%).

Conclusions:

• The developed one-pot, photo-initiated method offers a rapid and efficient approach for high-performance aptamer-affinity monolithic column preparation.
• This strategy overcomes limitations of traditional methods, enabling faster and more effective online specific recognition.
• The method shows promise for applications in food safety and environmental monitoring.