Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

499
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
499
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

18.6K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
18.6K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

796
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
796
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

11.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
11.3K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.4K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of glucose and insulin on HepG2-C3A cell metabolism.

Biotechnology and bioengineering·2010
Same author

Overexpression of antioxidant enzymes upregulates aryl hydrocarbon receptor expression via increased Sp1 DNA-binding activity.

Free radical biology & medicine·2010
Same author

Impact of hepatitis C viral replication on CD4+ T-lymphocyte progression in HIV-HCV coinfection before and after antiretroviral therapy.

AIDS (London, England)·2010
Same author

[Expression and diagnostic significance of CD34 in brain tumors of patients with refractory epilepsy].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2010
Same author

Characterization of pore-expanded amino-functionalized mesoporous silicas directly synthesized with dimethyldecylamine and its application for decolorization of sulphonated azo dyes.

Journal of hazardous materials·2010
Same author

Human leukocyte antigen-G (HLA-G) expression in cervical lesions: association with cancer progression, HPV 16/18 infection, and host immune response.

Reproductive sciences (Thousand Oaks, Calif.)·2010
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Related Experiment Video

Updated: Nov 5, 2025

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy
06:37

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

Published on: June 15, 2022

3.9K

Differential fluorescence microscopy by using a dynamic cylindrical-vector field.

Yanhui Cai, Wei Liu, Wenkai Yang

    Optics Letters
    |May 14, 2021
    PubMed
    Summary
    This summary is machine-generated.

    A new single-exposure Fluorescence Emission Difference (FED) microscopy technique uses dynamic polarization states to achieve super-resolution imaging. This method enhances speed and signal-to-noise ratio for advanced fluorescence microscopy applications.

    More Related Videos

    Conducting Multiple Imaging Modes with One Fluorescence Microscope
    08:32

    Conducting Multiple Imaging Modes with One Fluorescence Microscope

    Published on: October 28, 2018

    10.0K
    From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
    15:10

    From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

    Published on: October 9, 2014

    11.6K

    Related Experiment Videos

    Last Updated: Nov 5, 2025

    Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy
    06:37

    Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

    Published on: June 15, 2022

    3.9K
    Conducting Multiple Imaging Modes with One Fluorescence Microscope
    08:32

    Conducting Multiple Imaging Modes with One Fluorescence Microscope

    Published on: October 28, 2018

    10.0K
    From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
    15:10

    From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

    Published on: October 9, 2014

    11.6K

    Area of Science:

    • Optics and Photonics
    • Microscopy
    • Biophysics

    Background:

    • Fluorescence Emission Difference (FED) microscopy is an emerging super-resolution imaging technique.
    • Conventional FED microscopy relies on double-exposure and image subtraction to surpass the diffraction limit.
    • Existing methods face limitations in speed and signal-to-noise ratio for certain applications.

    Purpose of the Study:

    • To develop a novel, faster FED microscopy approach.
    • To achieve super-resolution imaging using a single-exposure scheme.
    • To enhance signal-to-noise ratio for improved fluorescence imaging.

    Main Methods:

    • Implementation of a single-exposure FED imaging scheme.
    • Utilizing dynamic cylindrical-vector fields for excitation beam polarization.
    • Switching between radial and azimuthal polarization states at high radio frequency.
    • Integration with lock-in amplifier detection.

    Main Results:

    • Achieved lateral spatial resolution of approximately lambda/4.
    • Demonstrated a single-exposure FED imaging capability.
    • Enabled high-speed fluorescence imaging with improved signal-to-noise ratio.

    Conclusions:

    • The proposed single-exposure FED microscopy offers a significant advancement over traditional methods.
    • This technique provides high spatial resolution and improved imaging performance.
    • It holds promise for various high-speed, high-SNR fluorescence imaging applications.