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Updated: Aug 6, 2026
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Monitoring Protein Adsorption with Solid-state Nanopores
Published on: December 2, 2011
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Nanopore sensing: A physical-chemical approach
Joseph W F Robertson1, Madhav L Ghimire2, Joseph E Reiner2
1Biophysical and Biomedical Measurement Group, Microsystems and Nanotechnology Division, Physical Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, United States of America.
Biochimica Et Biophysica Acta. Biomembranes
|May 14, 2021
Summary
Protein nanopores are powerful sensors for studying molecular transport and biopolymers. Their development, from early studies to modern single-molecule detection, is reviewed, focusing on pore chemistry and detection principles.
Area of Science:
- Biophysics
- Biochemistry
- Nanotechnology
Background:
- Protein nanopores are crucial for understanding membrane transport and biopolymer analysis.
- Early research utilized systems like the Coulter counter and squid axons.
Purpose of the Study:
- To review the historical development of protein nanopore sensors.
- To highlight modern applications in single-molecule detection.
- To explore the influence of pore chemistry on detection.
Main Methods:
- Literature review of historical and modern protein nanopore studies.
- Analysis of biophysical principles governing molecular detection.
- Focus on the chemical properties of biological pores.
Main Results:
- Protein nanopore sensors have evolved significantly from early concepts.
- Modern applications enable exquisite detection of minute volume changes and single molecules.
- The chemistry of protein pores fundamentally impacts detection capabilities.
Conclusions:
- Protein nanopore sensors offer advanced capabilities for biophysical and biochemical analysis.
- Understanding pore chemistry is key to optimizing molecular detection.
- These sensors are vital tools for single-molecule science.
