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Knockdown of Long Non-Coding RNA HCP5 Increases Radiosensitivity Through Cellular Senescence by Regulating

Cuihong Wang1, Guanying Yu2, Ying Xu1

  • 1Cancer Center, The Second Hospital of Shandong University, Jinan, Shandong, 250033, People's Republic of China.

Cancer Management and Research
|May 17, 2021
PubMed
Abstract

Insights

This study reveals that high HLA complex P5 (HCP5) levels increase glioma radioresistance by suppressing microRNA-128. Reducing HCP5 enhances radiosensitivity, suggesting HCP5 and microRNA-128 as potential targets for glioma therapy.

Area of Science:

  • Neuro-oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Glioma is the most common adult malignant brain tumor, with radioresistance posing a significant therapeutic challenge.
  • HLA Complex P5 (HCP5) is dysregulated in various cancers, but its role in glioma radiosensitivity remains unexplored.

Purpose of the Study:

  • To investigate the impact of HCP5 on the radiosensitivity of glioma cells.
  • To elucidate the molecular mechanisms underlying HCP5's influence on glioma radioresistance.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to measure HCP5 and microRNA (miR)-128 levels.
  • Cell proliferation assays and senescence-associated β-galactosidase (SA-β-Gal) staining to assess radiosensitivity and cellular senescence.
  • Luciferase reporter and RNA immunoprecipitation (RIP) assays to confirm the interaction between HCP5 and miR-128.

Main Results:

  • HCP5 expression was elevated in glioma tissues and cells, inversely correlated with miR-128 levels.
  • Knockdown of HCP5 significantly reduced glioma cell proliferation and enhanced radiosensitivity.
  • HCP5 directly targets and regulates miR-128, and its effect on radiosensitivity is mediated through this interaction.

Conclusions:

  • The interaction between long non-coding RNA (lncRNA) HCP5 and miR-128 regulates glioma cell radiosensitivity, potentially by affecting cellular senescence.
  • HCP5 and miR-128 represent promising therapeutic targets for overcoming radioresistance in glioma treatment.
Keywords:
HLA complex P5cellular senescencegliomamicroRNA-128radiosensitivity

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