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Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Related Experiment Video

Updated: Nov 5, 2025

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Published on: March 2, 2020

Recent progress in fluorescent probes for bacteria.

Yurou Huang¹, Weijie Chen¹, Jeewon Chung²

¹Key Laboratory of Pesticide and Chemical Biology, Ministry of education, Hubei International Scientific and technological cooperation Base of Pesticide and Green Synthesis, International Joint research center for Intelligent Biosensing Technology and Health, College of chemistry, Central China Normal University, Wuhan 430079, P. R. China and Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University, Wuhan 430062, P. R. China.

Chemical Society Reviews

|May 20, 2021

Summary

This summary is machine-generated.

Accurate bacterial monitoring is crucial for health. This review highlights recent advances in using fluorescence-based molecular probes for sensitive and specific bacterial detection and imaging.

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Area of Science:

Microbiology and Biotechnology
Chemical Engineering and Materials Science

Background:

Bacteria are vital in food fermentation, antibiotic production, and pollutant degradation.
Certain bacteria pose significant threats to human health, necessitating effective monitoring strategies.
Molecular probe and fluorescence imaging technologies offer noninvasive, highly specific, and sensitive bacterial detection methods.

Purpose of the Study:

To review recent advancements in bacterial detection utilizing fluorescence-based techniques.
To generalize common mechanisms for designing organic fluorescent probes for bacterial detection and imaging.
To provide a perspective on the future of fluorescent probes in bacterial monitoring.

Main Methods:

Review of recent literature on fluorescence-based bacterial detection.
Generalization of design principles for organic fluorescent probes.
Discussion of imaging techniques for bacterial analysis.

Main Results:

Fluorescence imaging with molecular probes is a powerful tool for bacterial detection.
Various mechanisms exist for designing specific and sensitive fluorescent probes.
The field shows significant progress in enhancing detection capabilities.

Conclusions:

Fluorescence-based methods are highly effective for bacterial detection and imaging.
Continued development of organic fluorescent probes will improve specificity and sensitivity.
This technology holds great promise for addressing challenges posed by pathogenic bacteria.