Related Experiment Video
Updated: Nov 4, 2025
12:23
In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
Published on: September 7, 2022
1.9K
Sensitive Detection of SARS-CoV-2 Using a SERS-Based Aptasensor.
Hao Chen1, Sung-Gyu Park2, Namhyun Choi1
1Department of Chemistry, Chung-Ang University, Seoul 06974, South Korea.
ACS Sensors
|May 21, 2021
Summary
A novel surface-enhanced Raman scattering (SERS) aptasensor offers highly sensitive detection of SARS-CoV-2. This platform achieves a low limit of detection for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in under 15 minutes.
Area of Science:
- Nanotechnology
- Biosensing
- Molecular Diagnostics
Background:
- Accurate and rapid detection of SARS-CoV-2 is crucial for pandemic control.
- Current diagnostic methods have limitations in sensitivity and speed.
- Development of advanced biosensors is needed for improved SARS-CoV-2 detection.
Purpose of the Study:
- To develop a highly sensitive aptasensor for quantifying SARS-CoV-2.
- To utilize surface-enhanced Raman scattering (SERS) for sensitive detection.
- To establish a rapid diagnostic platform for SARS-CoV-2 detection.
Main Methods:
- Developed a SERS-based aptasensor using a spike protein DNA aptamer as the receptor.
- Employed a self-grown gold (Au) nanopopcorn surface as the SERS detection substrate.
- Quantified SARS-CoV-2 by monitoring SERS peak intensity changes upon aptamer-target binding.
Main Results:
- Achieved high sensitivity in quantifying SARS-CoV-2 lysates.
- Demonstrated a limit of detection (LoD) below 10 PFU/mL.
- Completed detection within 15 minutes.
Conclusions:
- The developed SERS aptasensor platform enables sensitive and rapid SARS-CoV-2 detection.
- This technology shows potential for clinical applications, improving upon current diagnostic kits.
- The study highlights the feasibility of using aptasensors for improved viral detection accuracy.
