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Updated: Nov 4, 2025

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Single-Entity Detection With TEM-Fabricated Nanopores.

Hongcheng Yang1, Muhammad Saqib1, Rui Hao1

  • 1Department of Chemistry, Southern University of Science and Technology, Shenzhen, China.

Frontiers in Chemistry
|May 24, 2021
PubMed
Summary

Solid-state nanopores fabricated using transmission electron microscopy (TEM) offer sensitive detection for gene sequencing and molecule analysis. This technology promises high-speed, low-cost next-generation sequencing.

Keywords:
TEM fabricationelectron-beam drillingsequencingsingle entity detectionsolid-state nanopores

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Area of Science:

  • Nanoscience and Nanotechnology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Nanopore-based detection is crucial for advanced sensing and sequencing.
  • Solid-state nanopores provide durability, reusability, and tunable pore sizes.
  • Transmission electron microscopy (TEM) is key for fabricating and characterizing nanopores.

Purpose of the Study:

  • To review recent advancements in TEM-fabricated nanopore modification and characterization.
  • To highlight applications in detecting nucleic acids, proteins, and nanoparticles.
  • To explore the role of computer simulations in nanopore sequencing strategies.

Main Methods:

  • Fabrication of solid-state nanopores using transmission electron microscopy.
  • Characterization of nanopore properties and modifications.
  • Analysis of ionic current signals during single-entity translocation.

Main Results:

  • TEM-fabricated nanopores demonstrate high sensitivity for single-entity detection.
  • Successful applications shown in nucleic acid, protein, and nanoparticle analysis.
  • Progress in modifying and characterizing nanopores for enhanced performance.

Conclusions:

  • TEM-fabricated solid-state nanopores are vital for sensitive, high-speed, and cost-effective detection.
  • Future developments focus on computer simulations and addressing current challenges.
  • This technology is poised to advance next-generation sequencing.