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Updated: Nov 4, 2025
Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation
Published on: February 16, 2018
Isolation of adult mouse microglia using their in vitro adherent properties
Zoe Woolf1,2, Taylor J Stevenson2,3, Kevin Lee2,4
1Department of Pharmacology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, Private Bag 92019, New Zealand.
Abstract:
Microglia are the primary innate immune effectors of the central nervous system. Although numerous protocols have been developed to isolate fetal mouse microglia, the isolation of adult mouse microglia has proven more difficult. Here, we present a simple, widely accessible protocol to isolate pure microglia cultures from 4- to 14-month-old mouse brains using their adherent properties in vitro. These isolated microglia recapitulate the adherent properties of adult human microglia and present a more suitable model for studying age-related diseases. For complete details on the use and execution of this protocol in adult human microglia, please refer to Rustenhoven et al. (2016).
Insights
Researchers developed a new protocol to isolate pure adult mouse microglia, which are key immune cells in the brain. This method provides a better model for studying neurodegenerative and age-related diseases.
Area of Science:
- Neuroscience
- Immunology
- Cell Biology
Background:
- Microglia are the central nervous system's primary immune cells.
- Isolating adult mouse microglia is challenging compared to fetal microglia.
Purpose of the Study:
- To present a simple protocol for isolating pure adult mouse microglia.
- To establish a suitable model for studying age-related central nervous system diseases.
Main Methods:
- Utilized adherent properties of microglia in vitro.
- Isolated microglia from 4- to 14-month-old mouse brains.
Main Results:
- Successfully isolated pure microglia cultures from adult mouse brains.
- Isolated microglia exhibited adherent properties similar to adult human microglia.
Conclusions:
- The protocol provides a widely accessible method for obtaining adult mouse microglia.
- This model is valuable for research into age-related neurological conditions.
