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Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin
Published on: September 12, 2019
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Mouse Models of Intestinal Fibrosis
Jiannan Li1, Dina Dejanovic1, Megan T Zangara1
1Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH, USA.
Methods in Molecular Biology (Clifton, N.J.)
|May 24, 2021
Summary
Mouse models are crucial for studying intestinal fibrosis and testing treatments. This study details common models, including chemically induced, bacterial-induced, and spontaneous inflammation models, for research into fibrosis mechanisms and therapies.
Area of Science:
- Gastroenterology
- Immunology
- Animal Models
Background:
- Intestinal fibrosis is a significant complication of chronic inflammatory bowel diseases.
- Understanding the mechanisms driving fibrosis is crucial for developing effective treatments.
- Mouse models are vital tools for studying intestinal fibrosis and evaluating therapeutic strategies.
Purpose of the Study:
- To provide a comprehensive overview of established mouse models for intestinal inflammation and fibrosis.
- To detail protocols for commonly used and emerging models relevant to fibrosis research.
- To facilitate the selection and implementation of appropriate models for investigating disease mechanisms and therapeutic interventions.
Main Methods:
- Description of chemically induced colitis models: Dextran Sodium Sulfate (DSS) and 2,4,6-Trinitro-benzene Sulfonic Acid (TNBS).
- Inclusion of a bacterially triggered colitis model using Adherent-Invasive Escherichia coli (AIEC).
- Presentation of spontaneous intestinal inflammation models: Interleukin-10 knockout (IL-10KO) and SAMP/YitFc mice.
Main Results:
- Established protocols for multiple mouse models of intestinal inflammation and fibrosis are presented.
- These models recapitulate key aspects of human intestinal fibrotic diseases.
- The described models offer diverse approaches to study fibrosis pathogenesis.
Conclusions:
- A range of validated mouse models are available for studying intestinal fibrosis.
- These models are essential for dissecting disease mechanisms and testing novel therapeutic agents.
- Standardized protocols enhance reproducibility and comparability in fibrosis research.
