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Updated: Nov 4, 2025

Efficient Polyethylene Glycol PEG Mediated Transformation of the Moss Physcomitrella patens
Published on: April 19, 2011
Plastid Transformation in Physcomitrium (Physcomitrella) patens: An Update
1Graduate School of Informatics, Nagoya University, Nagoya, Japan. sugita@gene.nagoya-u.ac.jp.
Abstract:
The moss Physcomitrium (Physcomitrella) patens performs efficient homologous recombination in both the nucleus and plastid enabling the study of individual gene function by generating precise inactivation or modification of genes. Polyethylene glycol (PEG)-mediated transformation of protoplasts is routinely used to study the nuclear gene function of P. patens. PEG-mediated protoplast transformation is also applied for plastid transformation of this moss. The efficiency of plastid transformation is quite reliable and one or two homoplasmic transplastomic lines are obtained in a plastid transformation experiment (5 × 105 protoplasts) by selection for spectinomycin resistance.
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