Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Nov 4, 2025

Obtaining Eggs from Xenopus laevis Females
04:47

Obtaining Eggs from Xenopus laevis Females

Published on: August 20, 2008

14.9K

Obtaining Xenopus tropicalis Eggs.

Maura Lane1, Emily K Mis2, Mustafa K Khokha2

  • 1Pediatric Genomics Discovery Program, Department of Pediatrics and Genetics, Yale University School of Medicine, New Haven, Connecticut 06510, USA maura.lane@yale.edu.

Cold Spring Harbor Protocols
|May 25, 2021
PubMed
Summary

This protocol details frog egg collection for research. Xenopus tropicalis frogs are induced with hormones for egg collection, crucial for cell and developmental biology studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ccdc57 regulates cilia and left-right patterning in Xenopus.

Biology open·2026
Same author

Referral route: a determinant of inequity for children with undiagnosed genetic diseases?

Frontiers in genetics·2026
Same author

ReacStick: Evaluating Executive Function Independent From Level of Education.

American journal of physical medicine & rehabilitation·2025
Same author

Trio exome analysis is a valuable tool for genetic diagnosis of epilepsy in Mali.

Genetics in medicine open·2025
Same author

A yeast model reveals the SMAD3 nuclear import mechanism.

bioRxiv : the preprint server for biology·2025
Same author

Biallelic variants in the conserved ribosomal protein chaperone gene <i>PDCD2</i> are associated with hydrops fetalis and early pregnancy loss.

Proceedings of the National Academy of Sciences of the United States of America·2025

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Herpetology

Background:

  • Xenopus frogs are valuable model organisms for biological research due to year-round egg and embryo production.
  • Xenopus egg extracts serve as cell-free systems for studying cellular processes.
  • Xenopus embryos are ideal for developmental biology, integrating traditional embryology with genetic tools.

Purpose of the Study:

  • To provide a detailed protocol for collecting Xenopus tropicalis eggs.
  • To outline the necessary steps for hormone induction and egg retrieval in Xenopus tropicalis.
  • To highlight species-specific differences in egg collection procedures between Xenopus laevis and Xenopus tropicalis.

Main Methods:

  • Induction of Xenopus tropicalis females with a hormone to stimulate ovulation.

More Related Videos

Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis
06:05

Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

Published on: May 25, 2017

8.7K
Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs
10:01

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Published on: June 27, 2013

13.9K

Related Experiment Videos

Last Updated: Nov 4, 2025

Obtaining Eggs from Xenopus laevis Females
04:47

Obtaining Eggs from Xenopus laevis Females

Published on: August 20, 2008

14.9K
Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis
06:05

Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

Published on: May 25, 2017

8.7K
Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs
10:01

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Published on: June 27, 2013

13.9K
  • Collection of eggs following hormone administration.
  • Description of frog handling techniques required for Xenopus tropicalis egg collection.

    • Main Results:

    • Successful induction and collection of Xenopus tropicalis eggs.
    • Established a reproducible protocol for Xenopus tropicalis egg harvesting.
    • Detailed the practical aspects of handling Xenopus tropicalis for experimental procedures.

    Conclusions:

    • Xenopus tropicalis is a suitable model for genetic studies due to its diploid genome.
    • The protocol provides essential guidance for researchers working with Xenopus tropicalis in cell and developmental biology.
    • Proficiency in handling Xenopus tropicalis is key to successful egg collection and subsequent research.