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Klaus W Berndt^1,2, Ignacy Gryczynski¹, Joseph R Lakowicz¹

¹University of Maryland School of Medicine, Department of Biological Chemistry, 660 West Redwood Street, Baltimore, MD 21201.

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|May 25, 2021

Summary

This summary is machine-generated.

The Hamamatsu PLP-01 picosecond light pulser offers a cost-effective, compact alternative for frequency-domain fluorescence measurements. Despite lower power and longer pulse width, it enables high-frequency measurements and fluorophore mixture resolution.

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Area of Science:

Optics and Photonics
Spectroscopy

Background:

Frequency-domain fluorescence measurements are crucial for analyzing fluorophore lifetimes.
Traditional light sources like dye lasers can be expensive and complex.
There is a need for accessible and user-friendly excitation sources for fluorescence spectroscopy.

Purpose of the Study:

To evaluate the Hamamatsu PLP-01 picosecond light pulser as a 413-nm excitation source for frequency-domain fluorescence measurements.
To compare its performance and characteristics against conventional sync-pumped dye lasers.
To assess its suitability for resolving complex fluorophore mixtures.

Main Methods:

Utilized the Hamamatsu PLP-01 as a 413-nm excitation light source.
Conducted frequency-domain fluorescence measurements.
Compared the PLP-01's pulse width, repetition rate, output power, cost, and size to dye lasers.
Investigated time drifts between optical and electrical signals.

Main Results:

The PLP-01 has a longer Full Width at Half Maximum (FWHM) of 40 ps compared to dye lasers.
It offers a similar pulse repetition rate (up to 10 MHz) but lower output power.
Measurements up to 2000 MHz modulation frequency were achieved, enabling resolution of different fluorophore lifetimes.
Significant time drifts (up to 2 hours) were observed between optical and electrical signals.

Conclusions:

The PLP-01 is a more affordable, compact, and easier-to-handle alternative for fluorescence measurements.
It is capable of high-frequency measurements and resolving mixtures of fluorophores.
Ongoing work aims to address and eliminate observed time drifts.