Related Experiment Video
Updated: Aug 18, 2026
Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
Published on: June 28, 2013
Several intermediate forms in the processing of rat lutropin subunits as shown by sodium dodecyl sulphate
A Starzec1, R Counis, M Jutisz
1Laboratoire des Hormones Polypeptidiques, CNRS, Gif-sur-Yvette, France.
Abstract:
To study the biosynthesis in situ of lutropin (LH) subunits, anterior pituitary cells in culture were employed. The cells were incubated in the presence of [35S]methionine. Labelled polypeptides, immunologically related to alpha and LH beta subunits, were isolated by specific immunoprecipitation, then analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and revealed by fluorography. Electrophoretic analysis of immunoprecipitates is a crucial step in this methodology. It permits simultaneous purification, characterization and accurate quantitation of the radioactivity specifically incorporated into LH subunits. Also, using SDS-PAGE, it is possible to isolate and identify the different processed forms of LH subunits.
More Related Videos
07:40Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples
Published on: May 5, 2017
09:37Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
Published on: May 2, 2019