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Resolution of stereoisomers of dipeptides by gas chromatography on Chirasil-Val
B Koppenhoefer1, H Allmendinger, L P Chang
1Institut für Organische Chemie der Universität, Tübingen, F.R.G.
Journal of Chromatography
|May 27, 1988
Abstract:
Several dipeptides have been separated into stereoisomers (enantiomers, diastereomers, sequential isomers) by gas chromatography on Chirasil-Val. Sufficiently volatile N-trifluoroacetyl dipeptide methyl esters were formed at ambient temperature, thus avoiding racemization (or epimerization, respectively, and cleavage of the peptide bond. This method is suitable for ascertaining the stereoisomeric composition of dipeptides in biological samples.