Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Nov 4, 2025

A Microfluidic-based Hydrodynamic Trap for Single Particles
10:13

A Microfluidic-based Hydrodynamic Trap for Single Particles

Published on: January 21, 2011

16.9K

A microfluidic device enabling deterministic single cell trapping and release.

Huichao Chai1, Yongxiang Feng1, Fei Liang1

  • 1State Key Laboratory of Precision Measurement Technology and Instrument, Department of Precision Instrument, Tsinghua University, Beijing, China. wwh@tsinghua.edu.cn.

Lab on a Chip
|May 28, 2021
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Label-Free Dual-Mode Flow Cytometry for Single-Cell Physicochemical Property Analysis.

Analytical chemistry·2026
Same author

Dual-Branch Fusion Network: Precise Decoding of Lower Limb Multi-Joint Torque.

IEEE transactions on bio-medical engineering·2026
Same author

Atomic-scale mechanism unlocks thermal-stable high-κ performance in HfO<sub>2</sub> via coherent interfaces.

Nature communications·2026
Same author

Deficiency of extracellular vesicles miR-32 from bone marrow mesenchymal stem cells alleviates vascular calcification in type 2 diabetes by inhibiting endothelial ferroptosis.

Stem cell research & therapy·2026
Same author

Treadmill exercise alleviates Alzheimer's disease pathologies in APP/PS1 mice through modulation of microglial glucose metabolic reprogramming.

Frontiers in aging neuroscience·2026
Same author

Network pharmacology analysis and in vitro experimental validation of liriodenine against non-small cell lung cancer.

BMC cancer·2025
Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026
Same journal

A particulate blood-mimicking fluid with physiological biconcave geometry for microscale hemorheology.

Lab on a chip·2026
Same journal

Multicellular sensor arrays fabricated by capillary stamping for pattern-based odor discrimination.

Lab on a chip·2026
Same journal

A real-time microfluidic surveillance system for multiplex detection of heavy metal contamination in wastewater.

Lab on a chip·2026
Same journal

Vision-guided parallel manipulation of cells with optoelectronic tweezers.

Lab on a chip·2026
Same journal

Review of nanofluidic mass transport systems: engineering through physicochemical fields and interfacial properties.

Lab on a chip·2026
See all related articles

This study introduces a novel microfluidic device for precise single-cell isolation. The device enables high-efficiency cell trapping and deterministic, sequential release for advanced biological analysis.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • Single-cell isolation is crucial for detailed biological and chemical analysis.
  • Existing passive microfluidic devices often lack deterministic cell release capabilities.

Purpose of the Study:

  • To develop a novel microfluidic device for efficient and deterministic single-cell trapping and release.
  • To enable sequential isolation of individual cells for subsequent analysis.

Main Methods:

  • Design of a microfluidic device with an array of trapping sites, main channel, trigger channel, and air channel.
  • Integration of two types of capillary valves for automated, predefined valve opening.
  • Application of pressure from the trigger channel to achieve last-trapped-first-released (LTFR) cell elution.

More Related Videos

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip
07:05

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Published on: September 27, 2019

6.2K
Cell Capture Using a Microfluidic Device
29:02

Cell Capture Using a Microfluidic Device

Published on: October 1, 2007

5.6K

Related Experiment Videos

Last Updated: Nov 4, 2025

A Microfluidic-based Hydrodynamic Trap for Single Particles
10:13

A Microfluidic-based Hydrodynamic Trap for Single Particles

Published on: January 21, 2011

16.9K
Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip
07:05

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Published on: September 27, 2019

6.2K
Cell Capture Using a Microfluidic Device
29:02

Cell Capture Using a Microfluidic Device

Published on: October 1, 2007

5.6K
  • Development of a theoretical model for trapping and release flow fields.

    • Main Results:

    • Demonstrated high-efficiency, sequential trapping of cells into distinct sites.
    • Achieved deterministic release of individual trapped cells using the trigger channel mechanism.
    • Validated the device's performance using MCF-7 cells, confirming its capability for controlled single-cell manipulation.

    Conclusions:

    • The developed microfluidic device offers a robust platform for deterministic single-cell isolation and release.
    • This technology advances sample preparation for single-cell analysis, overcoming limitations of previous methods.
    • The device has potential applications in various fields requiring precise cell manipulation and analysis.