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Updated: Nov 4, 2025
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Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells
Published on: September 28, 2022
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Flow Cytometry Analysis of Mouse Hematopoietic Stem and Multipotent Progenitor Cells
Julien M P Grenier1,2, Marjorie C Delahaye1,2, Stéphane J C Mancini1,2
1Aix Marseille University, CNRS, INSERM, Institut Paoli-Calmettes, Cancerology Research Center of Marseille (CRCM), Marseille, France.
Methods in Molecular Biology (Clifton, N.J.)
|May 31, 2021
Summary
This study introduces a flow cytometry method for analyzing mouse hematopoietic stem and progenitor cells. The technique enables comparison of JAM-C protein expression across different cell subsets.
Area of Science:
- Hematology
- Immunology
- Cell Biology
Background:
- Flow cytometry is a standard technique for multiparametric analysis of single cells.
- Hematopoietic stem and progenitor cells (HSPCs) are crucial for blood formation and require precise characterization.
- Understanding protein expression in distinct HSPC subsets is vital for regenerative medicine and disease research.
Purpose of the Study:
- To develop and validate a novel flow cytometry-based method for analyzing HSPC subsets.
- To enable quantitative comparison of protein expression, specifically JAM-C, between different HSPC populations.
- To provide a tool for deeper investigation into the heterogeneity of the hematopoietic stem cell compartment.
Main Methods:
- Isolation of hematopoietic stem and progenitor cells from murine long bones.
- Application of multiparametric flow cytometry for single-event analysis.
- Quantification of JAM-C protein expression on identified HSPC subsets.
Main Results:
- Successful isolation and characterization of distinct HSPC subsets using flow cytometry.
- Demonstration of the method's capability to detect variations in JAM-C protein expression.
- Establishment of a reproducible protocol for analyzing JAM-C in HSPC populations.
Conclusions:
- The described flow cytometry method is effective for analyzing HSPC subsets.
- This approach facilitates the comparative analysis of JAM-C protein expression in HSPCs.
- The findings offer a valuable tool for future research in hematopoiesis and stem cell biology.
