Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ultrasonography01:17

Ultrasonography

6.8K
Ultrasonography is an imaging technique that uses high-frequency sound waves to visualize the body's internal structures. It is a non-invasive and safe procedure that does not involve the use of ionizing radiation, making it widely used in various medical fields. Ultrasonography is used to study heart function, blood flow in the neck or extremities, certain conditions such as gallbladder disease, and fetal growth and development.
During an ultrasonography procedure, a handheld device called...
6.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficacy and Safety Assessment of 5-Fluorouracil, Irinotecan and Oxaliplatin-Loaded Implants in Mouse and Pig Models for Pancreatic Cancer Therapy.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Additive-Free Edge-Functionalized Graphene Dough.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

A New Bioprinted Dual-Layered Corneal Structure Using Collagen-Based Bioinks.

Tissue engineering. Part A·2026
Same author

Corrigendum to "Ulvan structural modification enhances stability and cell compatibility of GelMa based bioinks for tissue engineering" [Int. J. Biol. Macromol. 321 (2025) 146461].

International journal of biological macromolecules·2026
Same author

Laser ablation as a rapid prototyping approach for fabricating metallic antennas on soft polymer substrates.

Journal of materials chemistry. B·2025
Same author

Implantable drug delivery system improves tolerability of anti-CD40/anti-PD1 and chemotherapy in a murine model of breast cancer.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2025

Related Experiment Video

Updated: Nov 3, 2025

Author Spotlight: A Stable Phantom Material for Optical and Acoustic Imaging
04:54

Author Spotlight: A Stable Phantom Material for Optical and Acoustic Imaging

Published on: June 16, 2023

3.3K

Reference Phantom Method for Ultrasonic Imaging of Thin Dynamic Constructs.

Andres Ruland1, James M Hill2, Gordon G Wallace1

  • 1ARC Centre of Excellence for Electromaterials Science (ACES), Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Wollongong, New South Wales, Australia.

Ultrasound in Medicine & Biology
|June 1, 2021
PubMed
Summary
This summary is machine-generated.

Quantitative ultrasound can monitor tissue engineering using the reference phantom method (RPM). This study validates RPM for dynamic hydrogels, showing accurate measurements despite sound speed and attenuation changes, simplifying construct evaluation.

Keywords:
Local attenuationQuantitative ultrasound imagingReference phantom methodSound speed diffractionTissue engineering

More Related Videos

Construction of a Preclinical Multimodality Phantom Using Tissue-mimicking Materials for Quality Assurance in Tumor Size Measurement
06:33

Construction of a Preclinical Multimodality Phantom Using Tissue-mimicking Materials for Quality Assurance in Tumor Size Measurement

Published on: July 29, 2013

11.5K
Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure
10:22

Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure

Published on: February 12, 2018

10.9K

Related Experiment Videos

Last Updated: Nov 3, 2025

Author Spotlight: A Stable Phantom Material for Optical and Acoustic Imaging
04:54

Author Spotlight: A Stable Phantom Material for Optical and Acoustic Imaging

Published on: June 16, 2023

3.3K
Construction of a Preclinical Multimodality Phantom Using Tissue-mimicking Materials for Quality Assurance in Tumor Size Measurement
06:33

Construction of a Preclinical Multimodality Phantom Using Tissue-mimicking Materials for Quality Assurance in Tumor Size Measurement

Published on: July 29, 2013

11.5K
Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure
10:22

Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure

Published on: February 12, 2018

10.9K

Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Ultrasound Imaging

Background:

  • Quantitative ultrasound (QUS) offers non-destructive evaluation of tissue-engineered constructs.
  • Local attenuation and integrated backscatter coefficient (IBC) monitor biological development.
  • Reference phantom method (RPM) determines these parameters but struggles with dynamic constructs (e.g., evolving sound speed, attenuation, thickness).

Purpose of the Study:

  • To assess the feasibility of using RPM for dynamic constructs, specifically biodegradable hydrogels developing neocartilage.
  • To validate an ultrasonic evaluation procedure using a 50-MHz transducer.
  • To compensate for significant attenuation and sound speed variations.

Main Methods:

  • Experiments conducted on 3-mm-thick acellular hydrogels with microspheres.
  • Validated ultrasonic procedure to detect and compensate for large attenuation variations (up to 20-fold).
  • Quantified integrated backscatter coefficient percentage change (IBCΔ) as a function of sound speed mismatch (ΔSS).

Main Results:

  • Estimation errors for local attenuation were below 1%, even with 20-fold attenuation variations.
  • Sound speed mismatch did not affect local attenuation but reduced backscatter intensity.
  • Developed an equation (IBCΔ = (0.63 ± 0.07) ΔSS + (8.54 ± 0.76) x 10⁻³ ΔSS²) to compensate for IBC reduction due to sound speed mismatch (up to 120 m/s), with <7% error.
  • Spectral difference method accurately measured depth mismatch; combined with sound speed mismatch, effects were negligible.

Conclusions:

  • The RPM can be adapted for evaluating thin dynamic constructs like hydrogels.
  • The developed compensation methods allow for accurate QUS parameter estimation despite significant construct property variations.
  • These findings enable the use of a generic reference phantom, simplifying QUS-based monitoring in tissue engineering.