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Development of a Glucose Sensor Based on Glucose Dehydrogenase Using Polydopamine-Functionalized Nanotubes
Won-Yong Jeon1, Hyug-Han Kim2, Young-Bong Choi2
1School of Chemical Engineering and Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, Suwon 16419, Korea.
Membranes
|June 2, 2021
Summary
This study developed a novel glucose sensor using polydopamine-functionalized carbon nanotubes to immobilize enzymes and mediators. This approach enables sensitive and selective glucose detection in biological samples, improving upon existing methods.
Area of Science:
- Electrochemistry and Biosensors
- Nanomaterials in Diagnostics
- Enzyme-Immobilization Techniques
Background:
- Electrochemical glucose detection is crucial for diagnostics, often relying on enzyme-mediated signal transduction.
- Glucose dehydrogenase (GDH) is preferred over glucose oxidase (GOx) due to its oxygen insensitivity.
- Existing Ru-based mediators primarily work with GOx, and improved electrode attachment for GDH-compatible mediators like Ru(dmo-bpy)2Cl2 is needed.
Purpose of the Study:
- To develop an effective method for attaching Ru(dmo-bpy)2Cl2 and GDH onto electrode surfaces for glucose sensing.
- To create a cross-linker-free immobilization strategy for enhanced sensor performance.
- To demonstrate selective glucose detection in complex biological matrices.
Main Methods:
- Utilized polydopamine-functionalized multi-walled carbon nanotubes (PDA-MWCNTs) for enzyme and mediator immobilization.
- Fabricated sensors on screen-printed carbon electrodes (SPCEs).
- Characterized materials using FT-IR, Raman, TGA, CV, and EIS; employed multi-potential step (MPS) technique for detection.
Main Results:
- Successfully attached Ru(dmo-bpy)2Cl2 and GDH onto SPCEs using PDA-MWCNTs without cross-linkers.
- Demonstrated a wide linear range for glucose concentration-dependent responses.
- Achieved selective glucose detection in the presence of interfering species and in biological fluids (serum) using MPS.
Conclusions:
- The PDA-MWCNT platform provides an effective, cross-linker-free method for immobilizing GDH and Ru(dmo-bpy)2Cl2.
- The developed sensor exhibits high performance, including selectivity and a wide linear range for glucose detection.
- This sensing strategy offers a viable pathway for advanced glucose sensors and is potentially adaptable for other bioanalytes.
