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Updated: Nov 2, 2025

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
Published on: December 30, 2016
An approach to fast 2D nuclear magnetic resonance at low concentration based on p-H2 -induced polarization and
Ruud L E G Aspers1, Marco Tessari1
1Institute for Molecules and Materials, Radboud University, Nijmegen, The Netherlands.
Abstract:
Recent developments in para-hydrogen-induced polarization (PHIP) methods allow the nuclear magnetic resonance (NMR) detection of specific classes of compounds, down to sub-micromolar concentration in solution. However, when dealing with complex mixtures, signal resolution requires the acquisition of 2D PHIP-NMR spectra, which often results in long experimental times. This strongly limits the applicability of these 2D PHIP-NMR techniques in areas in which high-throughput analysis is required. Here, we present a combination of fast acquisition and nonuniform sampling that can afford a 10-fold reduction in measuring time without compromising the spectral quality. This approach was tested on a mixture of substrates at micromolar concentration, for which a resolved 2D PHIP spectrum was acquired in less than 3 min.
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