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Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform
Published on: November 9, 2017
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Cytokines: From Clinical Significance to Quantification
Chao Liu1, Dewei Chu1, Kourosh Kalantar-Zadeh2
1School of Materials Science and Engineering, University of New South Wales, Sydney, NSW, 2052, Australia.
Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|June 11, 2021
Summary
Accurate cytokine quantification is vital for understanding immune responses and diagnosing diseases. This review covers methods, challenges, and advances in measuring these critical immune mediators for clinical and research applications.
Area of Science:
- Immunology
- Biochemistry
- Clinical Diagnostics
Background:
- Cytokines are key regulators of immune and inflammatory responses.
- Cytokine levels serve as crucial biomarkers for numerous diseases.
- Accurate quantification aids in understanding physiological/pathological processes, diagnosis, and treatment.
Purpose of the Study:
- To provide a comprehensive overview of cytokine quantification.
- To discuss factors influencing cytokine measurement in biological fluids.
- To summarize recent technological advancements and challenges in cytokine analysis.
Main Methods:
- Review of existing literature on cytokine quantification techniques.
- Discussion of various in vitro and in vivo assays.
- Comparison of different quantification platforms and commercially available assays.
Main Results:
- Cytokine quantification offers insights into pro- and anti-inflammatory effects.
- Factors like sample handling and storage significantly impact measurement accuracy.
- Advances include high-sensitivity assays, biosensors, and multiplex analysis.
Conclusions:
- Reliable cytokine quantification is essential for clinical medicine and biological research.
- Technological progress is driving towards real-time, point-of-care multiplex analysis.
- Overcoming current challenges will enhance diagnostic and therapeutic applications.
