Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of Fluorophore-Quencher Pairs for Distance-Dependent Molecular Sensing in Photoacoustic Imaging.

Chemical & biomedical imaging·2026
Same author

Temporal Programming of Cell-Free Transcription Using Orthogonal Enzyme-Responsive DNA Blockers.

ACS synthetic biology·2026
Same author

Tune, Extend, and Narrow the Useful Dynamic Range of Cell-Free Transcription Biosensors Through Programmable DNA-Based Stem-Loop Hairpin Reporters.

Angewandte Chemie (International ed. in English)·2026
Same author

Continuous, Week-Long, Seconds-Resolved <i>In Vivo</i> Drug Measurements Performed with a Xenonucleic Acid-Employing Electrochemical, Aptamer-Based Sensor.

Journal of the American Chemical Society·2026
Same author

Pilot phase clinical trial of a wearable, electrochemical aptamer-based patch for continuous drug concentration measurement.

Nature biotechnology·2026
Same author

Immune-Induced Antibody-DNA Hybrid Condensates.

Journal of the American Chemical Society·2025

Related Experiment Video

Updated: Nov 2, 2025

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
09:05

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

18.2K

Programmable, Multiplexed DNA Circuits Supporting Clinically Relevant, Electrochemical Antibody Detection.

Sara Bracaglia1, Simona Ranallo1,2, Kevin W Plaxco2

  • 1Department of Chemical Science and Technologies, University of Rome, Tor Vergata, 00133 Rome, Italy.

ACS Sensors
|June 15, 2021
PubMed
Summary

Researchers developed a novel electrochemical DNA circuit for rapid, sensitive antibody detection. This versatile platform can identify multiple specific antibodies, even in complex biological samples like serum.

Keywords:
DNA circuitsDNA nanotechnologyDNA sensorsantibody monitoringelectrochemical biosensors

More Related Videos

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

9.8K
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

14.6K

Related Experiment Videos

Last Updated: Nov 2, 2025

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
09:05

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

18.2K
Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

9.8K
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

14.6K

Area of Science:

  • Biotechnology and Biosensing
  • Molecular Diagnostics
  • Electrochemistry

Background:

  • Current health crises necessitate rapid, sensitive, and convenient antibody detection platforms.
  • Existing methods may lack the required speed, sensitivity, or specificity for diverse applications.

Purpose of the Study:

  • To design and validate a novel electrochemical DNA circuit for quantitative detection of specific antibodies.
  • To demonstrate the platform's sensitivity, specificity, selectivity, and versatility in detecting multiple antibody targets.

Main Methods:

  • Development of a synthetic antigen-conjugated nucleic acid strand-based electrochemical DNA circuit.
  • Utilizing strand displacement reactions triggered by specific antibody recognition to release a redox reporter.
  • Employing electrochemical detection to quantify the released reporter, indicating antibody presence and concentration.

Main Results:

  • Achieved a low nanomolar detection limit, demonstrating high sensitivity for antibody detection.
  • Confirmed specificity, with no signal observed in the presence of non-targeted antibodies.
  • Demonstrated selectivity in complex media, including 90% serum, and successful multiplexed detection of five different antibodies without crosstalk.

Conclusions:

  • The designed electrochemical DNA circuit offers a sensitive, specific, and versatile platform for antibody detection.
  • The programmable nature and multiplexing capability make it suitable for detecting clinically relevant antibodies in complex biological samples.