Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

17.2K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
17.2K
Immunoprecipitation01:20

Immunoprecipitation

6.7K
Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
6.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Risk of Thyroid Cancer in Children, Adolescents and Young Adults with Hashimoto Thyroiditis: a 27 Years Follow-up Study.

Hormone research in paediatrics·2026
Same author

Addison's disease in Italy: mortality and survival by etiology.

Journal of endocrinological investigation·2026
Same author

Screening practices for diabetic peripheral neuropathy in pediatric type 1 diabetes: a nationwide survey by the ISPED Diabetes Study Group.

Acta diabetologica·2026
Same author

Temporal Programming of Cell-Free Transcription Using Orthogonal Enzyme-Responsive DNA Blockers.

ACS synthetic biology·2026
Same author

Tune, Extend, and Narrow the Useful Dynamic Range of Cell-Free Transcription Biosensors Through Programmable DNA-Based Stem-Loop Hairpin Reporters.

Angewandte Chemie (International ed. in English)·2026
Same author

Adherence to growth hormone treatment in the transition age: A prospective observational multicenter study.

Journal of the Endocrine Society·2026
Same journal

A Lithium Superionic Conductor Softened by Nonmetal-Chlorine Chemical Bonds.

Journal of the American Chemical Society·2026
Same journal

A Ferrocene Metal-Ligand Triplet Diradical with a Terminal Iminyl Group Discovered by Time-Resolved Mid-Infrared Spectroscopy.

Journal of the American Chemical Society·2026
Same journal

Regulating Li-Ion Transport via Solvent and Ion Clustering Using Ternary Salts in Nonfluorinated Solvents for Extended Cyclability of Zero-Excess Lithium-Metal Batteries.

Journal of the American Chemical Society·2026
Same journal

Terahertz-Field-Induced Dissociation of Frenkel Excitons in Organic Semiconductors.

Journal of the American Chemical Society·2026
Same journal

Interplay between Slow Chirality Inversion and Slow Guest Uptake in a Triple-Helical Closed-Cage Metallocryptand.

Journal of the American Chemical Society·2026
Same journal

Controlled Sulfane Sulfur Delivery via Allyl Disulfide Rearrangement-Mediated Thiosulfoxide Formation.

Journal of the American Chemical Society·2026
See all related articles

Related Experiment Video

Updated: Jan 14, 2026

Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms
11:36

Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms

Published on: May 29, 2020

3.4K

Immune-Induced Antibody-DNA Hybrid Condensates.

Sara Scalia1, Marco Cappa2, Lorenzo Rovigatti2

  • 1Department of Chemical Sciences and Technologies, University of Rome, Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy.

Journal of the American Chemical Society
|October 23, 2025
PubMed
Summary
This summary is machine-generated.

Researchers combined DNA and antibody interactions to create programmable, reversible antibody-DNA condensates. This novel approach offers potential applications in sensing and drug delivery systems.

More Related Videos

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
08:57

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

Published on: October 6, 2019

10.6K
Detection of Anti-MDA5 Autoantibodies Using HeLa Cells and Immunocytochemistry with Light Microscopy
10:55

Detection of Anti-MDA5 Autoantibodies Using HeLa Cells and Immunocytochemistry with Light Microscopy

Published on: October 31, 2025

667

Related Experiment Videos

Last Updated: Jan 14, 2026

Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms
11:36

Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms

Published on: May 29, 2020

3.4K
Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
08:57

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

Published on: October 6, 2019

10.6K
Detection of Anti-MDA5 Autoantibodies Using HeLa Cells and Immunocytochemistry with Light Microscopy
10:55

Detection of Anti-MDA5 Autoantibodies Using HeLa Cells and Immunocytochemistry with Light Microscopy

Published on: October 31, 2025

667

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Materials Science

Background:

  • Phase separation is crucial for cellular organization.
  • DNA and antibody interactions are fundamental biological processes.
  • Controlling phase separation with synthetic components is an active research area.

Purpose of the Study:

  • To develop a method for inducing phase separation using both Watson-Crick base-pairing and antibody-antigen interactions.
  • To create stable, programmable antibody-DNA hybrid condensates.
  • To explore the potential of these condensates in sensing and drug delivery.

Main Methods:

  • Utilized antigen-conjugated star-shaped DNA motifs (nanostars) with DNA sticky ends.
  • Employed specific bivalent antibodies to recognize the conjugated antigens.
  • Investigated concentration-dependent phase separation and reversibility.
  • Validated findings with phase-field modeling and simulations.

Main Results:

  • Achieved specific and orthogonal antibody-induced phase separation of four different antigen-conjugated nanostars.
  • Observed concentration-dependent condensate formation starting at low nanomolar antibody levels.
  • Demonstrated reversible formation and dissolution of antibody-DNA condensates.
  • Phase-field modeling qualitatively and quantitatively reproduced experimental results.

Conclusions:

  • Combined Watson-Crick and antibody-antigen interactions effectively induce phase separation in antibody-DNA hybrid condensates.
  • These programmable condensates exhibit stability and reversibility.
  • The approach mimics natural cellular systems and offers versatility for applications in sensing and drug delivery.