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Updated: Nov 1, 2025
Bridging the Bio-Electronic Interface with Biofabrication
Published on: June 6, 2012
An enzymatic histamine biosensor based on a screen-printed carbon electrode modified with a chitosan-gold
Natha Nontipichet1, Suntisak Khumngern1, Jittima Choosang1
1Center of Excellence for Trace Analysis and Biosensor, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand; Division of Physical Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Abstract:
A histamine biosensor was developed based on a screen-printed carbon electrode modified with Prussian blue (PB) electrodeposited on multi-walled carbon nanotubes covered with a macroporous layer of chitosan-gold nanoparticles composite cryogel (CS-AuNPs Cry). With its high specific surface area and conductivity, CS-AuNPs Cry proved an excellent supporting material for diamine oxidase (DAO) immobilization. PB acted as a redox mediator to promote electron transfer between hydrogen peroxide and the electrode surface. The PB reduction current was measured during the hydrogen peroxide-releasing oxidation of histamine catalyzed by DAO. The proposed biosensor displayed two linear ranges: 2.50-125.0 µmol L-1 and 125.0-400.0 µmol L-1. The limit of detection was 1.81 µmol L-1. Reproducibility was good (RSD = 5.46%), operational stability high, long-term stability excellent, and selectivity good. The biosensor determined histamine levels in fish and shrimps with satisfactory recoveries, and the obtained results agreed with those obtained by ELISA.
