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Related Experiment Video

Updated: Nov 1, 2025

A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans
09:00

A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans

Published on: May 2, 2018

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Microfluidic-based imaging of complete Caenorhabditis elegans larval development.

Simon Berger1,2, Silvan Spiri1, Andrew deMello2

  • 1Department of Molecular Life Science, University Zürich, Winterthurerstrasse 190, 8057 Zürich, Switzerland.

Development (Cambridge, England)
|June 25, 2021
PubMed
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This study introduces a new microfluidic device for long-term live imaging of Caenorhabditis elegans across larval stages. The system maintains worm orientation and identity, enabling detailed observation of development and gene function studies.

Area of Science:

  • Developmental Biology
  • Microfluidics
  • Genetics

Background:

  • Microfluidic techniques for Caenorhabditis elegans (C. elegans) imaging have advanced.
  • Current methods face limitations in maintaining stable worm orientation during long-term imaging or offer only short-term immobilization.
  • There is a need for imaging solutions that support extended observation of C. elegans development without compromising worm viability.

Purpose of the Study:

  • To develop a novel microfluidic imaging method for parallel, long-term live imaging of C. elegans.
  • To maintain stable worm orientation and identity throughout multiple larval stages.
  • To enable detailed observation of developmental processes and facilitate genetic manipulation studies.

Main Methods:

  • Utilized an array of microfluidic trap channels to ensure stable worm orientation and allow for growth and molting.
Keywords:
C. elegansImagingLong-termMicrofluidics

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Last Updated: Nov 1, 2025

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  • Implemented an active hydraulic valve system for temporary immobilization during image acquisition.
  • Developed a microfluidic device for on-chip RNA interference (RNAi) experiments.

    • Main Results:

    • Achieved parallel live-imaging of C. elegans across multiple larval stages with consistent orientation and identity.
    • Acquired high-quality images with minimal impact on worm viability and developmental timing.
    • Successfully observed hypodermal seam and P-cell divisions, and the complete process of vulval development.
    • Demonstrated feasibility of on-chip RNAi by perturbing basement membrane breaching during anchor cell invasion.

    Conclusions:

    • The novel microfluidic device enables unprecedented long-term, high-resolution live imaging of C. elegans development.
    • This method supports detailed studies of cell division, morphogenesis, and gene function in C. elegans.
    • The system is suitable for observing complex developmental processes and performing genetic screens.