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Morris Water Maze Test: Optimization for Mouse Strain and Testing Environment

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Evaluation of Rodent Cage Processing Using Reduced Water Temperatures.

Mary M Walker¹, Dianne M Harrison¹, Toi A Collins¹

¹University Laboratory Animal Resources, The Ohio State University, Columbus, Ohio.

Journal of the American Association for Laboratory Animal Science : JAALAS

|June 29, 2021

Summary

Lowering cage wash temperatures to 140°F effectively sanitizes animal cages, reducing energy costs. This study demonstrates that 140°F wash and rinse cycles are sufficient for cleaning and disinfection.

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Area of Science:

Laboratory animal science
Infectious disease control
Environmental hygiene

Background:

Previous studies suggested 143-180°F for cage sanitization.
Older methods may not reflect modern washing technology or cleaning challenges.

Purpose of the Study:

To evaluate lower temperatures for effective cage sanitization.
To determine cost savings associated with optimized washing parameters.

Main Methods:

Tested 6 wash/rinse combinations (125°F, 140°F, 180°F) on soiled and autoclaved cages.
Used ATP detection and RODAC plates for microbial sampling.
Verified temperatures with data loggers.

Main Results:

140°F wash (450s) and rinse (50s) effectively cleaned and disinfected cages.
Lower temperatures resulted in significant annual energy savings ($21,867.08 per washer).

Conclusions:

140°F is sufficient for effective cage sanitization in animal facilities.
Optimized washing parameters reduce operational costs without compromising hygiene.